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Microsoft Word - Vazquez-Moreno21-06proof1.doc
Serum 40.0 ± 0.3 2.2 ± 0.1a
Ig fraction 10 ± 0.1 2.7 ± 0.1
Unadsorbed 32.4 ± 0.2 ND
(albumin fraction)
Unadsorbed 9 ± 0.2 1.7 ± 0.2 (IgM, IgA, IgG)
Adsorbed 7.6 ± 0.3 2.2 ± 0.1 ( Ig fraction )
Adsorbed 1 ± [...] with five bed volumes of 0.5 M Na2SO4, 10 mM MOPS, pH 7.6 (Buffer A) and samples of 5 mL of porcine serum (adjusted to 0.5 M Na2SO4, 10 mM MOPS, pH 7.6) loaded onto column. The gel was washed with Buffer [...] eluted with 10 mM MOPS Buffer, pH 7.6 (Buffer B). Following elution, HA- Sepharose was cleaned with two bed volumes of 4 M guanidine hydrochloride (guanidine- HCl), pH 7.6; washed with five bed volumes of …